The Chromatography Forum

of Delaware Valley

Modern SPE-based Chromatographic Techniques for Protein and Peptide Analysis

Extracting analyte of interest from different biological matrices and delivering them to detection in a rapid and reliable yet simple fashion is critical for assay development, particularly in clinical and pharmaceutical research. Solid phase extraction (SPE) has made fundamental contributions to the analytical science, and is continuously evolving with advanced technologies in the context of sorbents, automation, and miniaturization. When it comes to protein and peptide analysis, liquid chromatography coupled with mass spectrometry (LCMS) has been the driving force of proteomics research. However, SPE-based separations are playing increasingly important roles in protein and peptide sample preparation. This seminar will discuss the critical role of the modern SPE and its evolution toward microextraction techniques, which are pivotal for addressing the challenges of complex biological matrices in biological and biomedical research. It will also discuss how the synergy between these advanced SPE techniques and LCMS is enabling high-sensitivity, high-throughput analysis for low-cell or single-cell proteomics.