The Chromatography Forum

of Delaware Valley

Analysis of proteins and metabolites by mass spectrometry has made extraordinary advances in the past 10 years, however improvements to the number of molecules measured has not led to equivalent improvements in translated biomarkers to the clinic.  We believe that translation of human proteomics and metabolomics studies to validated biomarkers has been limited in general by poor experimental design at the earliest stages of discovery. Some typical limitations include low sample numbers and generally a lack of quality control (QC) along the discovery pipeline, including the steps of sample preparation, analytical data collection, and data analysis.  In metabolomics, more attention has been paid to the number of features measured as opposed compound identity or analytical quantification. Our lab has focused on using our own mistakes along with first principles of analytical chemistry to revisit the biomarker pipeline and subsequently improve the quality of these discovery experiments. We have undertaken this with the goal of performing biomarker discovery in clinically relevant sample cohorts with methods that are more easily translatable to a clinical laboratory, hopefully to decrease the rate of biomarker attrition along the translational pipeline.  This presentation will use examples from the areas of hepatitis/liver fibrosis, cardiovascular disease, and sepsis to describe important sample preparation, separation, and mass spectrometry workflows and also pitfalls along the biomarker pipeline.

Reservations: Please register on-line at http://www.cfdv.org where you can pay by PayPal. You can also register by e-mailing Sheree Gold at sheree@cfdv.org indicating your name, employer and work telephone. Alternatively, you can telephone Ms. Gold at 610-742-4981 and provide this same information. Please note that “no shows” will be billed for the dinner.

Late reservations: We still want you to attend, so please call or email Ms. Sheree Gold at 610-742-4981 or sheree@cfdv.org. However, we cannot guarantee your entrée selection for dinner.

Non-weather-related cancellations:  The deadline for ‘good weather’ cancellations is 36 hours prior to the meeting. Cancellations after the deadline cannot be accepted and ‘no shows’ will be billed for dinner if it was not already paid in advance. 

Inclement weather cancellation policy: Cancellations will be accepted up to 12:00 pm on the day of the meeting. Call Ms. Sheree Gold at 610-742-4981. If the meeting is cancelled by the CFDV, you will be notified by email by 3pm.