The Chromatography Forum

of Delaware Valley

Abstract:

The signal enhancement benefits provided by capillary liquid chromatography columns are advantageous when sample volumes are naturally small, such as in bioanalytical investigations, or when analyte concentrations are low. For these benefits to be truly achieved, on-column focusing techniques are essential.  Solvent-based focusing is effective with capillary columns, but it is limited to a single band-compression step and can only be performed at the inlet.  This presentation will discuss advancements in temperature-assisted solute focusing (TASF).  In this technique, a linear array of independently controlled thermoelectric cooling elements generates dynamic temperature changes along the length of the column.  The head of the column is cooled to sub-ambient temperatures during injection to induce focusing, followed by rapid heating to the separation temperature.  This can then be repeated with sequential focusing zones for a multiplicative compression effect.  Simulations capable of predicting temperature effects on retention allow for rapid screening of experimental conditions, and a reduction in method development time.  Recent work to evaluate this technique will be presented, along with applying TASF to biological separations, and efforts to scale the technology up to commercially available column diameters.

A Zoom Teleconference Presentation

Please Note:  The CFDV continues to observe health and safety restrictions for all members and other parties interested in the separation sciences during the COVID-19 pandemic.  We are also committed to promotion and development in all aspects of separation sciences by sharing our professional experiences with the community.  To fulfill these commitments, we will conduct this presentation by video teleconference.  Our selected platform for this event will be Zoom. 

When it will be Presented:

Thursday, September 23, 2021, 12 pm ET (Expected to run approximately one hour.)

You will be able to access Zoom as an attendee at no cost.  However, you must register to receive the Zoom meeting ID and password.