The Chromatography Forum

of Delaware Valley

Abstract:
Due to excellent separation capacity for complex mixtures of chemicals, comprehensive two- dimensional gas chromatography (GCxGC) is being utilized with increasing frequency for untargeted metabolomics analysis, especially for volatile metabolites. Typically, when compared to one dimensional gas chromatography (GC), three-fold to ten-fold more peaks are detectable using GCxGC, which has enabled the rapid expansion of volatile metabolite catalogs for microbes, plants, animals, and humans. However, the large number of metabolites that are revealed by GCxGC analyses creates computational challenges, especially in studies that include dozens to hundreds of samples that must be aligned prior to analysis. In studies of fewer samples, the large peak tables that GCxGC can yield cause a statistical conundrum, wherein the number of metabolites are one or two orders of magnitude larger than the number of samples, which puts statistical models at risk of overfitting. Thus, the end goal of turning the large quantities of GCxGC data into biological information can be difficult to reach. In this presentation I'll provide a few concrete examples of how our use of GCxGC has
facilitated the characterization of an amazingly complex bacterial volatile metabolome, the challenges we've faced in aligning and interpreting the data, and some of the strategies we're currently employing to tackle the reams of data in order to find relationships between chemical and biological phenomena.

A Zoom Teleconference Presentation
Please Note: The CFDV continues to observe health and safety restrictions for all members and other parties interested in the separation sciences during the COVID-19 pandemic. We are also committed to promotion and development in all aspects of separation sciences by sharing our professional experiences with the community. To fulfill these commitments, we will conduct this presentation by video teleconference. Our selected platform for this event will be Zoom.

When it will be Presented:
Wednesday, December 9, 2020, 12 pm ET
Expected to run approximately one hour.

No Cost for this presentation.
You will be able to access Zoom as an attendee for no cost to either Zoom or the CFDV. However, you must register in order to receive the Zoom meeting ID and password.