The Chromatography Forum

of Delaware Valley

Over the past 15 years, a variety of advances in liquid chromatography columns and instrumentation have enabled improved capabilities for measuring pharmaceutical and biological analytes in a broad array of sample types. Initial studies on the impact of viscous friction in columns packed with superficially porous particles have guided the development of high-throughput LC methods for isocratic analysis of pharmaceutical compounds and gradient analysis of neurotransmitters. Strategies for packing capillary LC columns have been applied in microfluidic SPE-MS platforms for monitoring cellular lipid secretion and in integrated column cartridges for portable LC analysis. Data acquisition devices based on microcontrollers and single-board computers have been expanded to control capillary-scale pumping devices and regulate voltage for electrospray ionization in LC-MS applications. This broad array of strategies is now being expanded to improve the characterization of biopharmaceutical compounds and small molecule metabolites in clinical samples.