The Chromatography Forum

of Delaware Valley

Discounts available for multiple attendees from the same organization; for more information, contact joefoley888@gmail.com.

In 2DLC, sample components are fractionated by two different columns utilizing different retention mechanisms. To achieve successful 2D resolution of complex sample components, dissimilar (orthogonal) retention mechanisms are required to effectively spread the peaks throughout the available separation space.

In this course we will discuss in detail:

* Theory of 2DLC, from a practical point of view

* Instrumentation, including commercially available instruments

* Method development across a wide variety of sample types

* Applications to lipids, peptides, proteins, small and large molecule pharmaceuticals, biological extracts, industrial polymers, and surfactants

* Helpful insights that are important for achieving good results in the lab

Students are expected to be familiar with HPLC.

Those who take this course will learn background information essential to understanding the technique and achieve practically useful results with commercial instrumentation. Many aspects of 2DLC are shared with one-dimensional HPLC such as column technologies, pumps, solvent systems, and matching the detector. However, 2DLC has some issues which are not present in one-dimensional HPLC, and these will be explained in detail so that course participants will have this knowledge prior to starting method development. We will also explore the suitable instrumentation for 2DLC, and how to process data external to the acquisition software.

Applications of comprehensive 2DLC will be shown for complex industrial and biological samples, as well as simpler applications such as column switching, target peak purity investigation, and biopolymer analysis using commercial two-dimensional chromatographic instruments.