The Chromatography Forum

of Delaware Valley

Overview

Maximizing chromatographic efficiency requires the ability to properly operate an HPLC instrument and quickly identify and solve chromatography problems that arise. This intensive one-day short course, taught by a practicing chromatographer, highlights routine system operation and its role in preventing future problems, and offers tips for finding solutions to real-world chromatography problems. The morning session introduces a general philosophy for troubleshooting, and we will discuss what to look for when reviewing chromatograms. In addition, we will provide an in-depth overview of peak integration in chromatography. This is an underappreciated aspect of data analysis that can result in significant analytical errors if not used properly.

The afternoon session goes into more detail on linking symptoms with causes. This discussion makes extensive use of case studies and examples to help students learn by examining real-world problems and solutions. This workshop provides practical technical information that is not available from any other single source. You can benefit from the diverse content in this course regardless of your experience level.

Finally, we finish with the famous “LC Quiz,” a group problem-solving discussion where we explore numerous real-world problems and work through the steps necessary to solve each issue. Students usually remark that this is their favorite part of the class, thanks to the stories attached to many of the problems.

MORNING SESSION

- A General Approach to Organized Troubleshooting

          - Why are we here?

          - How do we get started with troubleshooting?

- General Troubleshooting Strategies

          - Troubleshooting concepts

- How to Read a Chromatogram

- Basic chromatography parameters

          - Measuring instrumental and chromatographic parameters

- Integration advice for poorly resolved peaks.

AFTERNOON SESSION

- LC Troubleshooting

          - Design-related problems in LC

          - Routine maintenance of mobile phases, pumps, columns, and detectors

          - Linking symptoms and solutions with the LC Troubleshooting Matrix

- Best Practices in Chromatography

          - A practical guide to preventing future problems.

- The LC Quiz

- Open Discussion: Bring your own examples and questions.

GENERAL DETAILS

Breakfast and lunch will be provided to attendees as part of the registration cost. Additionally, course registrants will receive free registration to the 2025 CFDV Symposium to be held the following day at the same location. 

PLEASE NOTE: A minimum of 10 participants are required for this course to be held. If 10 participants are not registered by April 1, 2025, the course will be canceled and refunds will be provided.