Rapid Analysis of Biological Samples using Microfluidic Capillary Electrophoresis Coupled to Mass Spectrometry Detection

with Erin Redman
Tuesday, December 5, 2017
Registration Has Ended
About This Meeting

Capillary electrophoresis (CE) has been demonstrated to be effective for analyzing a wide variety of biological samples, from monoclonal antibodies to small molecule metabolites. When interfaced with mass spectrometry (MS) analysis the integrated techniques present a powerful platform for characterizing complex biological samples. Over the past several years we have developed a microfluidic capillary electrophoresis (CE)-MS platform based on the use of glass microfluidic devices. These have advantages over the traditional fused silica capillary format in that the use of microfluidic technology provides seamless integration of the sample injection, separation, and electrospray ionization on a monolithic device. With this approach extremely fast and efficient separations can be reproducibly achieved and the inherent speed and efficiency of CE separations can begin to be realized. In this talk results will be presented using a commercially available microfluidic CE-MS interface (908 Devices Inc.) to characterize a diverse array of biological samples. Multilevel characterization of monoclonal antibody (mAb) based therapies will be described. The microfluidic CE-MS interface can be used to perform separations of intact mAb charge variants to assess charge heterogeneity and obtain mass information about the modifications to the protein. Near baseline separation of intact charge variants differing in net charge by as little as +1 can be achieved with total run times under 5 minutes. More site-specific information can be obtained by performing reduced and limited proteolysis of the mAbs to generate fragments of lower mass that shed light on region specific modifications. Another area where CE excels is the analysis of small polar molecules. The microfluidic CE-MS interface can perform very rapid separations of small molecules. In as little as 2 minutes the 20 natural amino acids are separated and analyzed with peak widths of ~0.55s. Results will be presented quantifying small molecule metabolites in biofluids, such as serum and spent growth media.

  • Pricing
    • Registration: Non-Student $30
    • Registration: Student $15
  • Event Times
    • 05:00 PM Executive Committee Meeting
    • 06:00 PM Social Hour
    • 06:30 PM Dinner
    • 07:30 PM Presentation
  • Location
  • Meal Options
    • Sauteed Chicken Marsala With Kennett Square Mushrooms
    • Grilled Pesto Salmon
    • Vegetarian Option